RSUP Dr. Soeradji Tirtonegoro Klaten was established on December 20, 1927, jointly by the estates (plantations) owned Dutch East Indies (now Indonesia), which consists of tobacco, sugarcane, and jute plantations.

Hospital is named "Dr. Scheurer Hospital" and managed by the foundation "Zending" engaged in the welfare of the people. Hospital led by Dr. Bakker.

In 1942 Japan entered and mastered the Indian Government of the Netherlands so that "Dr. Scheurer Hospital" is also controlled by Japan. In 1945, Japan lost the war and Indonesia proclaimed independence. Thus, "Dr.Scheurer Hospital" is also located on the power of the government of Indonesia and since that time it had been renamed to be Tegalyoso Klaten General Hospital. The name was taken from the name of the village where Dr. Soeradji Tirtonegoro was stayed. During the reign of Japan, the hospital was led by Dr. Maeda and Dr. Curuta. Then after the Japanese left (in 1945), the hospital was led by Dr. Soenoesmo.

In the transition from hospital under the management of the Government Hospital Zending RI there are still some foreign medical doctors, among others, Dr. Horner and Dr. Bakker Junior. During that time all employees RSUP Dr. Soeradji Tirtonegoro given the opportunity to choose, keep working at RSUP Dr. Soeradji Tirtonegoro for later appointed as civil servants or moved to another hospital Zending the Bethesda Hospital in Yogyakarta or Surakarta Jebres Hospital.

In 1952, Dr. Soenoesmo which at that time as head of the hospital, died karen appendicitis pain and underwent surgery. In lieu of the leadership of Dr Dr. Soeradji Tirtonegoro appointed Dr. Horner was accompanied by Dr. Junior Bakker.

In the Year 1954 RSUP Dr. Soeradji Tirtonegoro led Dr. Soepaat Soemosoedirdjo and since 1945 Dr Dr. Soeradji Tirtonegoro in full has been managed by the Ministry of Health (MOH).

In 1947, Dr. Soeradji Tirtonegoro also used for the lecture and practice by the Medical Faculty (PTK/Perguruan Tinggi Kedokteran), so from now on also RSUP Dr. Soeradji Tirtonegoro except for carrying out health and education services for Midwives and Nurses who have been there also used as a doctor of education.

PTK in RSUP Dr. Soeradji Tirtonegoro was in 1950 moved to Yogyakarta, which then grew and developed into the Medical Faculty of University of Gajah Mada Yogyakarta.

Therefore until now RSUP Dr. Soeradji Tirtonegoro Klaten establish close cooperation with the Medical Faculty of Gadjah Mada University in Yogyakarta. Cooperation with the Faculty of Medicine was formally confirmed in writing in 1975 based on the decision of the Minister of Health, among others, stipulates that RSUP Dr. Soeradji Tirtonegoro, together with Dr RS. Sardjito Yogyakarta is one of the practice for medical students Medical Faculty of Gadjah Mada University in Yogyakarta.

In 1978 declared Surat Keputusan Menteri Kesehatan RI Nomor : 134/Menkes/SK/IV/78 dated 28 April 1978 on the Organisational Structure and Working Procedures of the General Hospital, which among others provides that RSUP Dr. Soeradji Tirtonegoro Hospital is a Class C, the General Hospital are carrying out health services in at least 4 (four) branches of specialization are: Medicine, Surgery, Obstetrics & Gynecology and Pediatrics.

In 1992 RSUP Dr. Soeradji Tirtonegoro defined as a Hospital Unit Swadana With Terms, by the Minister of Health with Keputusan Menteri Kesehatan RI nomor 746/Menkes/SK/I/1992 dated September 2, 1992. With the stipulation as Swadana Unit, Dr Dr. Soeradji Tirtonegoro berweanang to manage / use functional reception directly.

In 1993, by Keputusan Menteri Kesehatan RI nomor 1168/Menkes/SK/XII/1993 dated December 15, 1993, Dr Dr. Soeradji Tirtonegoro class up from class C to Class B non Education.

In 1994, by letter number: S-733/MK.03/1994 dated October 6, 1994, Finance Minister stated that RSUP Dr. Soeradji Tirtonegoro be approved as a Hospital Unit Swadana Unconditionally. Followed later by Keputusan Menteri Kesehatan RI nomor 1285/Menkes/SK/XII/1994 dated December 28, 1994 regarding the determination RSUP Dr. Soeradji Tirtonegoro be Self-financing Hospital Units (Unconditionally).

In 1997 declared Undang-undang Nomor 20 tentang Penerimaan Negara Bukan Pajak (PNBP) are followed up with Peraturan Pemerintah Nomor 22 Tahun 1997 tentang Jenis dan Penyetoran PNBP then RSUP Dr. Soeradji Tirtonegoro including State Revenue Agency.

During the long period of time and after going through various changes in management towards the Hospital in accordance with changing times, then based on Surat Keputusan Menteri Kesehatan RI No. 1442 A/ Menkes/SK/XII/1997 dated 20 December 1997 set the name of a department of the Hospital Dr. Soeradji Tirtonegoro. Dr. Soeradji Tirtonegoro is one of the leaders in the movement of association Boedi Utomo and served as a doctor in the area of Klaten.

In 2001 by Keputusan Menteri Kesehatan RI Nomor : 934/Menkes/IX/2001 at 5 September 2001, Dr Dr. Soeradji Tirtonegoro Klaten approved as a teaching hospital for the FK-UGM (Medical Faculty of Gadjah Mada University) and serve as the Laboratory Centre for Development of Basic Essential Medical Services.

In 2003 by Keputusan Menteri Kesehatan RI Nomor 1594/Menkes/SK/XII/2002 dated December 27, 2002 RSUP Dr. Soeradji Tirtonegoro Klaten Hospital designated as a Class B Education.

In 2007 based on Keputusan Menteri Keuangan Nomor: 273/KMK.05/2007 tanggal 21 Juni 2007 and Keputusan Menteri Kesehatan RI Nomor 756/MenKes/SK/VI/2007 dated June 26, 2007 set RSUP Dr. Soeradji Tirtonegoro Klaten as hospital having Financial Management in General Services Agency.